Improved methods for the isolation and study of the C18, C20 and C22 monoethylenic fatty acid isomers of biological samples: Hg adducts, HPLC, AgNO3‐TLC/FID, and ozonolysis

Publisher Website
Google Scholar
Abstract
The monoethylenic isomers of C18, C20 and C22 chain lengths of the depot fat of a nonhominid primate (cynomolgus monkeys,Macaca fascicularis), fed a partially hydrogenated herring oil (IV=76.0) for 30 months, were examined by 2 different approaches. The first isolation method involved preparative gas liquid chromatography and argentation thin layer chromatography (TLC). The second sequence involved a chain‐length fractionation system based on the TLC of the methoxy‐bromomercuri quence involved a chain‐length fractionation system based on the TLC of the methoxy‐bromomercuri adducts of the total methyl esters to isolate groups of acids of common degrees of unsaturation, and then high performance liquid chromatography on a reverse‐phase column. In both cases, the monoethylenic isomer distribution was determined by ozonolysis in BF3/MeOH. Comparable results were obtained with the 2 methods. The second approach is recommended for small biological samples, especially for those containing a relatively high proportion of di‐ and other polyethylenic isomers which might interfere.