Plasmid CpG Depletion Improves Degree and Duration of Tumor Gene Expression After Intravenous Administration of Polyplexes
Open Access
- 4 March 2008
- journal article
- research article
- Published by Springer Nature in Pharmaceutical Research
- Vol. 25 (7) , 1654-1662
- https://doi.org/10.1007/s11095-008-9558-7
Abstract
Tumor gene expression after the intravenous (i.v.) administration of current polymer-based gene delivery systems is generally low and short-lived. Immune stimulatory CpG dinucleotides, present within the plasmid DNA of the polyplexes are likely to contribute to this. The effect of CpG replacement on the levels of transgene expression was studied, after the i.v. administration of polyethylenimine (PEI) polyplexes.Keywords
This publication has 57 references indexed in Scilit:
- Improved anti-cancer effect of interferon gene transfer by sustained expression using CpG-reduced plasmid DNAInternational Journal of Cancer, 2007
- Cellular Uptake of Cationic Polymer-DNA Complexes Via Caveolae Plays a Pivotal Role in Gene Transfection in COS-7 CellsPharmaceutical Research, 2007
- Non-viral adiponectin gene therapy into obese type 2 diabetic mice ameliorates insulin resistanceJournal of Controlled Release, 2006
- Genomic DNA methylation: the mark and its mediatorsPublished by Elsevier ,2006
- Macrophage activation by a DNA/cationic liposome complex requires endosomal acidification and TLR9-dependent and -independent pathwaysJournal of Leukocyte Biology, 2004
- CpG Methylation of a Plasmid Vector Results in Extended Transgene Product Expression by Circumventing Induction of Immune ResponsesMolecular Therapy, 2004
- Adaptive and innate immune responses to gene transfer vectors: role of cytokines and chemokines in vector functionGene Therapy, 2003
- Overcoming the Inflammatory Toxicity of Cationic Gene VectorsJournal of Drug Targeting, 2002
- Identification of methylated CpG motifs as inhibitors of the immune stimulatory CpG motifsGene Therapy, 2001
- Effect of liposome-encapsulated clodronate pretreatment on synthetic vector-mediated gene expression in miceGene Therapy, 1999