Calibration of Coulter Counters for Particles ∼1 μ in Diameter

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Abstract
A linear relationship between particle volume and instrument response is confirmed for particles between 0.75 and 2.5 μ3, but this relationship may not hold for smaller particles. The implicit assumption of negligible surface conductance in derivations of this relationship is found acceptable for polystyrene spheres about 1 μ in diameter. A potential source of error in calibration is identified and allowed for. A relationship is developed between observed count and true count which allows coincident passage correction to be based on all available experimental points. The critical volume is found experimentally to be a function of particle volume.