Preparation and Characterization of Intact Globular Cell Wall Peptidoglycan fromMicrococcus lysodeikticus
- 1 July 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 43 (7) , 1485-1492
- https://doi.org/10.1080/00021369.1979.10863661
Abstract
The intact globular cell wall peptidoglycan was prepared from Micrococcus lysodeikticus without any mechanical disruption. The purification procedure consists mainly of proteolytic digestion and extractions with hot 5% TCA and with 0.01 N NaOH. The purified preparation showed an amphoteric, heteroporous three-dimensional network structure of the peptidoglycan, which amounted to 77% of the dry weight of the preparation, retaining the chemical and morphological integrity as judged by an electron microscopic observation and chemical analyses.This publication has 7 references indexed in Scilit:
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