Regulation of prolactin and somatotropin mRNAs by thyroliberin.
- 1 March 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (3) , 1294-1298
- https://doi.org/10.1073/pnas.75.3.1294
Abstract
The tripeptide thyroliberin (thyrotropin-releasing hormone, TRH) regulates the levels of cytoplasmic prolactin and growth hormone (somatotropin) mRNA in a line of functional rat pituitary tumor cells. Intracellular mRNA levels were determined with specific complementary DNA probes in RNA-excess hybridization reactions and independently by 2-dimensional gel electrophoretic analysis of the products of mRNA-directed cell-free translation. Prolactin mRNA sequences increased from 1.1-4.5% of the cytoplasmic poly(A)-containing mRNA within 48 h after addition of TRH to the medium; in parallel, growth hormone mRNA sequences were decreased. Similar effects of prolactin mRNA levels were observed in cells grown in serum-containing medium or when the cells were maintained in a serum-free medium for 72 h prior to addition of TRH, suggesting that TRH is responsible for the stimulation of prolactin mRNA levels rather than for exerting a permissive effect for another factor present in serum.This publication has 24 references indexed in Scilit:
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