Invited Review: Regulation of myosin phosphorylation in smooth muscle
- 1 July 2001
- journal article
- review article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 91 (1) , 497-503
- https://doi.org/10.1152/jappl.2001.91.1.497
Abstract
Phosphorylation of the regulatory light chains of myosin II (rMLC) by the Ca2+/calmodulin-dependent myosin light-chain kinase (MLCK) and dephosphorylation by a type 1 phosphatase (MLCP), which is targeted to myosin by a regulatory subunit (MYPT1), are the predominant mechanisms of regulation of smooth muscle tone. The activities of both enzymes are modulated by several protein kinases. MLCK is inhibited by the Ca2+/calmodulin-dependent protein kinase II, whereas the activity of MLCP is increased by cGMP and perhaps also cAMP-dependent protein kinases. In either case, this results in a decrease in the Ca2+ sensitivity of rMLC phosphorylation and force production. The activity of MLCP is inhibited by Rho-associated kinase, one of the effectors of the monomeric GTPase Rho, and protein kinase C, leading to an increase in Ca2+sensitivity. Hence, smooth muscle tone appears to be regulated by a network of activating and inactivating intracellular signaling cascades.Keywords
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