AN ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) FOR INFECTIOUS BURSAL DISEASE VIRUS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 45  (1) , 51-55
Abstract
The application of the indirect enzyme-linked immunosorbent assay (ELISA) for detection of infectious bursal disease virus antibodies in chicken serum was investigated. The test procedure involved the coating of concentrated infectious bursal disease virus antigen onto polystyrene tubes, followed by the addition of chicken anti-infectious bursal disease virus serum and horseradish peroxidase labeled rabbit anti-chicken globulin. As an indicator substrate, 5-aminosalicylic acid, with the oxidant H2O2 was added. The reaction was stopped by 3 M NaOH and the color intensity of the reaction mixtures read in a spectrophotometer at 449 nm. The ELISA test was found to be a precise sensitive and reproducible means of measuring infectious bursal disease virus antibodies in chicken and turkey sera.