Comparison of the restriction endonuclease maps of unintegrated proviral DNAs from four xenotropic murine leukemia viruses

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Abstract
From purified linear and superhelical DNA, the restriction endonuclease maps of 4 xenotropic murine leukemia virus DNA from NFS, NZB, BALB/c and AKR mice were determined with 10 restriction endonucleases. Each xenotropic proviral DNA had a unique restriction endonuclease map, with differences in the gag, pol, env and terminal repeated sequence regions. Type-specific SacI and EcoRI sites in the env region were identical in all 4 xenotropic murine leukemia virus DNA and were not found in ecotropic murine leukemia virus DNA. Comparison of the xenotropic murine leukemia virus DNA maps with maps of ecotropic murine leukemia virus DNA showed that the pol- and terminal-repeated sequence regions were highly conserved. Other similarities in ecotropic and some xenotropic viral DNA suggest common origins.