Acute-Phase Proteins Before Cerebral Ischemia in Stroke-Prone Rats
- 1 March 2001
- journal article
- research article
- Published by Wolters Kluwer Health in Stroke
- Vol. 32 (3) , 753-760
- https://doi.org/10.1161/01.str.32.3.753
Abstract
Background and Purpose—A high degree of proteinuria has been reported in stroke-prone spontaneously hypertensive rats (SHRSP). We studied the effect of salt loading on the detailed protein pattern of serum and urine in 3 rat strains: Wistar-Kyoto, spontaneously hypertensive rats, and SHRSP, an inbred animal model for a complex form of cerebrovascular disorder resembling the human disease. Methods—Rats were given a permissive diet and received 1% NaCl in drinking water. The protein pattern in body fluids was assessed over time by 2-dimensional electrophoretic analysis. Brain alterations were monitored by MRI and histology. Results—Several proteins were excreted in urine after weeks of treatment and in advance of stroke: transferrin, hemopexin, albumin, α2-HS-glycoprotein, kallikrein-binding protein, α1-antitrypsin, Gc-globulin, and transthyretin. Markers of an inflammatory response, including very high levels of thiostatin, were detected in the serum of SHRSP at least 4 weeks before a stroke occurred. Conc...Keywords
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