SUBMICROSCOPIC HISTOCHEMICAL DEMONSTRATION OF INTRACELLULAR REACTIVE ZINC IN β CELLS OF PANCREATIC ISLETS

Publisher Website
Google Scholar
Abstract
Three submicro-scopic procedures for Zn-detection in [beta] cells of the islets of Langerhans of adult pigs, rabbits and rats, were studied. Procedure A was an intravenous method with dithlzone. In procedure B a modified Voigt''s .silver method was used. Procedure C involved a paraffin-embedding method. The electron microscope was used for sections prepared by all 3 methods. The above procedures for detection of zinc in [beta] cells were easily reproducible except Procedure C, in which considerable amounts of reduced Ag grains were extracted during dehydration. Procedure B proved disadvantageous because intracellular structures of [beta] cells were markedly distintegrated. Procedure A showed the sites of Zn and the preservation of [beta] cell ultrastructure most satisfactorily in the earliest period after dithizone injection. The reaction products, which correspond to the submicroscopic localizing sites of zinc, were demonstrated mainly in mature granules and on their encasing membranous sacs. A small amount were also present occasionally in relation to the endoplasmic reticulum.

This publication has 0 references indexed in Scilit: