Isolation and Culture of Protoplasts from Apple Callus and Cell Suspension Cultures
Open Access
- 1 May 1984
- journal article
- Published by American Society for Horticultural Science in Journal of the American Society for Horticultural Science
- Vol. 109 (3) , 348-350
- https://doi.org/10.21273/jashs.109.3.348
Abstract
Protoplasts were isolated from actively growing callus and cell suspension cultures initiated from apple leaf discs. Callus and cell suspension cultures incubated in a solution of 0.7 m mannitol, 0.01 m CaCl2, 2% cellulysin, 0.5% macerase yielded protoplasts from about 75% of the callus cells and 90% of the suspension cells. Protoplast viability was 95–98% upon isolation, and 80–85% after a 2-week culture in a modified Murashige and Skoog (MS) medium. About 60% of the protoplasts regenerated cell walls, and 5–10% of the original protoplasts exhibited cell division.Keywords
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