EFFECTS OF ACTINOMYCIN-D ON CELL-CYCLE KINETICS AND DNA OF CHINESE-HAMSTER AND MOUSE MAMMARY-TUMOR CELLS CULTIVATED INVITRO
- 1 January 1976
- journal article
- research article
- Vol. 36 (10) , 3654-3658
Abstract
Actinomycin D produces changes in the cell cycle kinetics of Chinese hamster [ovary] (HA1) and mouse mammary tumor cells (EMT6) cultivated in vitro. There was a reduced rate of progression of cells through S phase and a G2 arrest, the duration and degree of which were drug dose dependent. The lethal effects of the drug on the 2 cell lines were comparable. At the molecular level, DNA single strand breaks (true breaks and/or alkali labile bonds) appear initially. After drug removal, the extent of disappearance of these DNA strand breaks was drug dose dependent. Similar results were obtained for the 2 cell lines. At the lower drug concentration (0.1 .mu.g/ml), although the DNA strand breaks appeared to disappear after drug free incubation, about 60% of this cell population was not viable.This publication has 6 references indexed in Scilit:
- Radiation-Induced Mitotic Delay in Sea Urchin Eggs Treated with Puromycin and Actinomycin DRadiation Research, 1966
- Life Cycle Analysis of Mammalian CellsBiophysical Journal, 1966
- Chromosome aberrations induced by thymidineExperimental Cell Research, 1966
- Synthesis of RNA and protein required for the mitosis of mammalian cellsExperimental Cell Research, 1964
- Actinomycin and Nucleic Acid FunctionProgress in Nucleic Acid Research and Molecular Biology, 1964
- Effect of Actinomycin D on Cellular Nucleic Acid Synthesis and Virus ProductionScience, 1961