Ultraviolet Microscopy of X-Irradiated Intestine2

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Abstract
The color-translating ultraviolet microscope was used to study radiation effects in the mouse intestine. Generative cells showed pronounced changes in light absorption at 263 mµ; the light-absorbing clumps normally present in the nucleus disappeared. Later, diffuse, strong absorption was seen in cytoplasm and nucleus; still later, there was very little absorption. The earlier changes occurred uniformly in all generative cells and thus cannot be related to a stage in the mitotic cycle; the late changes were independent of resumption of mitosis. The principal cells had similar but much less pronounced changes in 263 mµ absorption. Later, changes occurred that were related to abnormal development. The Paneth cells showed similar changes in 263 mµ absorption. In addition, they showed early degeneration of a specific (secretory) cytoplasmic structure, followed by speedy recovery.