Transcription of sea urchin histone genes in Escherichia coli
Open Access
- 25 August 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (16) , 3889-3906
- https://doi.org/10.1093/nar/9.16.3889
Abstract
DNA fragments comprising units of the repeated histone genes from the sea urchins Psammechinusmiliaris and Echinusesculentus were placed under the control of bacteriophage λ promoters by cloning into λ replacement vectors. Although promoter-like regions exist within the cloned fragments, transcription of the histone genes is controlled mainly, but not exclusively, by λ PL promoter. A transcription map of the cloned P. miliaris histone DNA fragment was obtained. The order of histone genes in E. esculentus was deduced from electron microscopic analyses of heteroduplexes with P. miliaris histone genes, and is similar to that in P. miliaris. Translation products of the transcripts have not been found in E. coli.Keywords
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