MONOCLONAL ANTIBODIES TO CARCINOEMBRYONIC ANTIGEN (CEA): CHARACTERIZATION AND USE IN A RADIOIMMUNOASSAY FOR CEA

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Abstract
Fusion of immune spleen cells of mice immunized with CEA [carcinoembryonic antigen] with mouse myeloma [PS/NSI/I-Ag4-1] cells resulted in several hybrid cell lines secreting antibodies to CEA as tested by radioimmunoassay. Four of these were cloned by limiting dilution and large amounts of anti-CEA antibodies were produced by growing the anti-CEA-producing cell clones i.p. in mice and collecting the ascites fluids formed. Ascites fluids containing antibodies with both high and low affinity were obtained. The antibody having the highest affinity was specific to CEA, whereas 1 antibody showed cross-reaction with the normal cross-reacting antigen. The establishment of a competitive-inhibition double antibody radioimmunoassay with high sensitivity and specificity using mouse monoclonal antibodies is described.