Effect of EDTA on the Solid Phase Clq Immune Complex Assay
- 1 January 1982
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 67 (3) , 275-277
- https://doi.org/10.1159/000233030
Abstract
A solid-phase Clq immune complex (IC) assay (C1q-SP) is described. Wells of microtiter plates are coated with Clq, sera diluted 1:40 are added, and complexed IgG bound to the solid phase is detected by peroxidase-labeled protein A. This assay selectively binds aggregated human gammaglobulin and soluble IC prepared in vitro. Treatment of sera with EDTA prior to Clq-SP analysis interferes with the test.Keywords
This publication has 1 reference indexed in Scilit:
- Isolation of a Thermolabile Serum Protein which Precipitates -Globulin Aggregates and Participates in Immune Hemolysis.Experimental Biology and Medicine, 1961