THE AMINO ACID NUTRITION OF YEAST IN RELATIONSHIP TO BIOTIN DEFICIENCY

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Abstract
The effect of various amino acids, either alone or in combination, upon the growth of Saccharomyces cerevisiae, Fleischmann strain 139, under conditions of biotin deficiency was investigated. Although aspartic acid alone is stimulatory under these conditions, the combination of aspartic acid, trypto-phan, arginine, leucine, and methionine is necessary in order to allow growth equivalent to that in the presence of all amino acids. The amino acids tyrosine, isoleucine, glycine, valine, phenyl-alanine, alanine, histidine, and glutamic acid are of most importance in replacing aspartic acid but have essentially no effect in its presence.

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