The Translation in vitro of mRNA for Immunoglobulin Heavy Chains
Open Access
- 1 July 1973
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 36 (1) , 1-7
- https://doi.org/10.1111/j.1432-1033.1973.tb02877.x
Abstract
The addition of mRNA preparations to a number of heterologous cell‐free systems has been shown to result in the synthesis of immunoglobulin light chains. This paper reports the results of experiments in which an mRNA fraction prepared from MOPC 21 mouse myeloma cells has been used to stimulate the synthesis of immunoglobulin heavy chains in rabbit reticulocyte lysates. The synthesis of heavy chains in a homologous cell‐free system containing either microsomes or membrane‐derived polysomes is also described. The synthetic product has been characterised by finger‐printing in each case. The results reveal the presence of a unique methionine‐containing peptide in digests of the product of the rabbit reticulocyte cell‐free system. This peptide is not observed in corresponding digests of heavy chains synthesized in vivo or in the homologous cell‐free system in vitro.Keywords
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