IMMUNO‐HISTOCHEMICAL LOCALIZATION OF MYOGLOBIN IN HUMAN MUSCLE TISSUE AND EMBRYONAL AND ALVEOLAR RHABDOMYOSARCOMA

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Abstract
An immunohistochemical investigation of the presence and localization of myoglobin was performed on cardiac and skeletal muscle tissue and on 9 embryonal and 9 alveolar rhabdomyosarcomas, utilizing an immunoperoxidase technique. Cardiac muscle fibers were evenly stained whereas staining of skeletal muscle fibers varied, giving a mosaic-like pattern. Of the fixatives used (4% formaldehyde, 4% paraformaldehyde, formaldehyde-glutaraldehyde, and Bouin''s fixative), 4% formaldehyde gave the most prominent staining; short fixation-time slightly increased the staining intensity. All the 9 embryonal and 9 alveolar rhabdomyosarcomas contained positively-stained tumor cells. The number of cells positively-stained and the intensity of the staining varied with differentiation: the most differentiated rhabdomyoblasts stained most intensely. Many poorly-differentiated tumor cells in highly cellular areas were positive. Myoglobin is considered a suitable marker of rhabdomyoblastic differentiation, thus the method used in this study may be valuable in the diagnosis.