l-2,3-Diaminopropionate: One of the building blocks for the biosynthesis of Zwittermicin A inBacillus thuringiensissubsp.kurstakistrain YBT-1520
- 8 August 2008
- journal article
- Published by Wiley in FEBS Letters
- Vol. 582 (20) , 3125-3131
- https://doi.org/10.1016/j.febslet.2008.07.054
Abstract
Zwittermicin A (ZwA) is a hybrid polyketide–non‐ribosomal peptide that is thought to be biosynthesized from five proposed building blocks, including the 2,3‐diaminopropionate. Candidate genes for de novo biosynthesis of 2,3‐diaminopropionate, zwa5A and zwa5B, have been identified in a previous study. In this research, zwa5A was interrupted and chemically synthesized 2,3‐diaminopropionate was used to feed the zwa5A − mutant. Results showed that feeding with 2,3‐diaminopropionate restored the ability of the zwa5A − mutant to produce ZwA. Another non‐ribosomal peptide synthase gene, designated orf3, was identified. Amino acid dependent PPi release assay showed that the adenylation domain ZWAA2 of ORF3 acyl‐adenylated l‐2,3‐diaminopropionate effectively. Taken together, it can be concluded that l‐2,3‐diaminopropionate is indeed one of the building blocks for the biosynthesis of Zwittermicin A.Keywords
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