Sequencing and characterization of pST1, a cryptic plasmid from Streptococcus thermophilus

15 August 1992

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 95 (2-3) , 175-180
https://doi.org/10.1016/0378-1097(92)90425-n

Abstract

Eighty-six strains of S. thermophilus were examined for their plasmid content. Thirteen strains were found to contain one or two plasmids ranging in size from 2.1 to 7.4 kb. DNA-DNA hybridization analysis revealed the presence of five distinct groups of DNA homology. The complete nucleotide sequence of plasmid pST1 (Accession number [X65856][1]), which belongs to the major homology group, was determined. It has a molecular size of 2093 bp, a GC content of 35% and contains one major open reading frame of 945 bp (ORF A). The predicted protein, designated Rep A, showed sequence homology with replication proteins from a group of plasmids which are known to replicate via single-stranded DNA intermediates (ssDNA plasmids). [1]: /lookup/external-ref?link_type=GEN&access_num=X65856&atom=%2Ffemsle%2F95%2F2-3%2F175.atom

Keywords

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