Microspectrophotometric Determination of Deoxyribonucleic Acid in Primary and Metastatic Mouse Mammary Tumors

Abstract
A microspectrophotometric study of the relationship of ploidy to metastasis is described. Five spontaneous and 11 metastatic mouse mammary tumors were used. The 11 metastatic tumors included 9 intravenously transplanted and 2 spontaneous. All the metastatic tumors tended to have a higher ploidy than the primary tumors from which they were derived. Diploid cells, although present in the primary tumors, were absent from some metastases. However, the presence of diploid cells in some metastatic tumors suggested that diploid as well as polyploid cells were able to leave the primary tumor and establish themselves at metastatic foci.

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