IMMUNOLOCALIZATION OF COMPLEMENT COMPONENT-C9 ON NECROTIC AND NON-NECROTIC MUSCLE-FIBERS IN MYOSITIS USING MONOCLONAL-ANTIBODIES - A PRIMARY ROLE OF COMPLEMENT IN AUTOIMMUNE CELL-DAMAGE

  • 1 January 1984
    • journal article
    • research article
    • Vol. 52  (1) , 181-188
Abstract
Monoclonal antibodies to human complement C9 were used to localize the membrane attack complex (MAC) of human complement on muscle fibers from patients with autoimmune myositis. A pigeon erythrocyte model system was used to screen the available monoclonal antibodies for their ability to bind C9 after its insertion into the MAC. Of the 5 antibodies tested, 3 were demonstrated, using radioiodine- or rhodamine-labeled 2nd antibody, to bind. The antibody giving maximum binding in this system was used to demonstrate that MAC were present on histologically normal as well as on necrotic fibers in myositis. No binding was seen in muscle from control subjects. These results suggest a primary role of complement in the pathogenesis of muscle fiber necrosis in myositis.

This publication has 28 references indexed in Scilit: