A compact semi-automated continuous cultivation system for Plasmodium falciparum
- 1 January 1981
- journal article
- Published by Oxford University Press (OUP) in Transactions of the Royal Society of Tropical Medicine and Hygiene
- Vol. 75 (2) , 292-298
- https://doi.org/10.1016/0035-9203(81)90338-2
Abstract
A compact inexpensive semi-automated continuous cultivation system for Plasmodium falciparum is described. The system is simple to operate and allows cultures to proceed unattended for several days. A particularly useful feature is a portable cultivation module that is freely transferable from the incubator to a tissue culture cabinet for manual operations such as sampling or addition of red cells. Each cultivation module holds plastic flasks which can give a combined culture surface area of up to 500 cm2. Parasitaemias of 12% are attained routinely at red cell densities of 1 to 7 x 10(8) per ml, if the medium is changed daily.Keywords
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