Alpha-Subunit Positions Methionine 180 and Glutamate 214 of Pseudomonas stutzeri OX1 Toluene- o -Xylene Monooxygenase Influence Catalysis
Open Access
- 15 February 2005
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (4) , 1511-1514
- https://doi.org/10.1128/jb.187.4.1511-1514.2005
Abstract
Alpha-subunit position M180 of toluene- o -xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o -cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p- nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.Keywords
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