Dynamics of extracellular release of Aspergillus fumigatus DNAand galactomannan during growth in blood and serum
Open Access
- 1 April 2010
- journal article
- research article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 59 (4) , 408-413
- https://doi.org/10.1099/jmm.0.017418-0
Abstract
Aspergillus fumigatus is the major cause of invasive aspergillosis (IA), a disease associated with high rates of morbidity and mortality in patients undergoing treatment for haematological malignancies. This study investigated A. fumigatus growth in vitro and in a murine model of IA in order to provide insights into the dynamics of extracellular DNA and galactomannan (GM) release and their relevance to early diagnosis of IA. Following inoculation of whole blood with 20 A. fumigatus conidia ml−1, DNA that corresponded to the inoculum could be detected by PCR but GM was not detected in plasma separated from the blood sample, indicating that the fungus did not grow in whole blood. The quantities of DNA detected by PCR, and GM, were proportional to the amount of fungal biomass present in vitro. Fungal DNA could be detected in the sera of mice experimentally infected with A. fumigatus with maximum detection in cyclophosphamide-treated mice.Keywords
This publication has 22 references indexed in Scilit:
- Use of PCR for diagnosis of invasive aspergillosis: systematic review and meta-analysisThe Lancet Infectious Diseases, 2009
- Value of Serial Quantification of Fungal DNA by a Real-Time PCR-Based Technique for Early Diagnosis of Invasive Aspergillosis in Patients with Febrile NeutropeniaJournal of Clinical Microbiology, 2009
- Real‐time PCR on the first galactomannan‐positive serum sample for diagnosing invasive aspergillosis in liver transplant recipientsTransplant Infectious Disease, 2008
- Revised Definitions of Invasive Fungal Disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus GroupClinical Infectious Diseases, 2008
- Differential Roles of Poly- N -Acetylglucosamine Surface Polysaccharide and Extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis BiofilmsApplied and Environmental Microbiology, 2008
- In Vitro Release by Aspergillus fumigatus of Galactofuranose Antigens, 1,3-β- d -Glucan, and DNA, Surrogate Markers Used for Diagnosis of Invasive AspergillosisJournal of Clinical Microbiology, 2006
- Use of Quantitative Real-Time PCR To Study the Kinetics of Extracellular DNA Released from Candida albicans , with Implications for Diagnosis of Invasive CandidiasisJournal of Clinical Microbiology, 2006
- Diagnosis of fungal infection: new technologies for the mycology laboratoryTransplant Infectious Disease, 2002
- Serum Stimulates Growth of and Proteinase Secretion byAspergillus fumigatusInfection and Immunity, 2002
- Quantitative PCR Assay To Measure Aspergillus fumigatus Burden in a Murine Model of Disseminated Aspergillosis: Demonstration of Efficacy of Caspofungin AcetateAntimicrobial Agents and Chemotherapy, 2001