The effect of silicate cement on the mitochondria and lysosomes of cultured cells assessed by quantitative enzyme histochemistry

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Abstract
Summary: The cytotoxic effect of the silicate cement ‘Silicap’ was evaluated in an in vitro system which simulates the clinical usage of the material. BHK‐21 (C‐13) cells on cover slips were exposed to the freshly mixed material for 1h, and stained for the demonstration of succinic dehydrogenase and acid phosphatase. The mean stain density for succinic dehydrogenase and acid phosphatase in the experimental cells was 77% and 153% of the control values respectively. The mechanism of cytotoxicity of ‘Silicap’ is discussed.
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