Production and Isolation of Large Quantities of Monoclonal Antibody Using Serum-Free Medium and Fast Protein Liquid Chromatography
- 1 April 1989
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 8 (2) , 241-247
- https://doi.org/10.1089/hyb.1989.8.241
Abstract
A method for the production and purification of monoclonal antibody on a large laboratory scale is described. It involves the growth of monoclonal antibodies in serum-free medium, ultrafiltration through an Amicon XM100A filter and fast protein liquid chromatography on a mono Q column with an ionic strength and pH elution gradient. Up to 30 mg of antibody per litre of cell culture supernatant was purified in one day. The procedure can be carried out at 4°.This publication has 12 references indexed in Scilit:
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