Synthesis of Oligo-ApGp and Other Oligonucleotides by Ribonuclease N1*
- 1 July 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 70 (1) , 55-61
- https://doi.org/10.1093/oxfordjournals.jbchem.a129626
Abstract
1. The synthesis of IpN, ApGpU, IpApApC and oligo-ApGp by ribonuclease N1 [ribonucleate guaninenucleotido-2′-transferase (cyclizing), Neurospora crassa, EC 2. 7. 7. 26] was studied. 2. The yield of IpC amounted to 22% with respect to phosphate donor when I-cyclic-p and cytidine were incubated with ribonuclease N1 at 4°C. The yield of IpN was dependent on the nature of phosphate acceptor in the following order: cytosine > uridine > adenosine > guanosine. ORD curve of IpC showed a characteristic multi-cotton effect due to dimer formation. The hypochromicity of IpC was 8.9% at pH 7.0, μ = 0.1. 3. The yield of ApGpU and IpApApC synthesized by ribonuclease N1 amounted to 39% and 6.5%, respectively. 4. Oligo-ApGp was synthesized by ribonuclease N1 and fractionated by DEAE-Sephadex column chromatography in the presence of 7 M urea. The yields of tetra-nucleotide and hexanucleotide fraction were 33% and 7%, respectively.Keywords
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