DNA polymerase III-dependent repair synthesis in response to bleomycin in toluene-treated Escherichia coli
- 1 October 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 179 (3) , 595-605
- https://doi.org/10.1007/bf00271750
Abstract
Bleomycin (BLM) is an antitumor drug which interacts with and damages DNA. We have reported a repair response dependent on DNA polymerase I in toluene-treated Escherichia coli. We report here that DNA polymerase III can also catalyze a repair response in toluene-treated E. coli following exposure to BLM. Polymerase III-mediated synthesis differs because it is ATP-dependent, whereas polymerase I-mediated repair synthesis is not. Polymerase III repair synthesis is independent of replicative synthesis, as demonstrated in a polA -, dnaB ts strain, or use of Novobiocin to inhibit replication, and replication persists in the presence of repair synthesis. It appears that ATP-dependent repair synthesis in response to BLM is also present in polA + strains. Repair synthesis does not require the uvrA gene product.This publication has 54 references indexed in Scilit:
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