Removal of 3’Untranslated Sequences Dramatically Enhances Transient Expression of Ovine Follicle‐Stimulating Hormone Beta Gene Messenger Ribonucleic Acid
- 1 December 1992
- journal article
- Published by Wiley in Journal of Neuroendocrinology
- Vol. 4 (6) , 655-658
- https://doi.org/10.1111/j.1365-2826.1992.tb00215.x
Abstract
The influence of the 3'untranslated (3'-UT) region of the ovine follicle-stimulating hormone (FSH) β mRNA on the level of transcript expression was studied. Only very low levels of FSH β mRNA were detected following transient transfection of COS cells with a eukaryotic expression vector containing a full-length ovine FSH β cDNA that includes 1.1 kilobases (kb) of 3'-UT. In contrast, deletion of all but 135 basepairs (bp) of the 3'-UT resulted in a striking increase in FSH β mRNA expression following transfection of the truncated cDNA construct. These observations suggest that sequences within the 3'-UT of the ovine FSH β mRNA may play a significant role in the post-transcriptional regulation of FSH β expression.Keywords
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