Monoclonal antibodies against metalloporphyrins. Specificity of interaction with structurally different metalloporphyrins
- 5 December 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 355 (3) , 314-316
- https://doi.org/10.1016/0014-5793(94)01221-0
Abstract
Monoclonal antibodies against Pd-coproporphyrin I have been obtained. The antibody specificity for free as well as for conjugated Pd-coproporphyrin I is characterized. Affinity constants are estimated for 3 monoclonal antibodies effectively interacting with free Pd-coproporphyrin I. A comparative study on the binding of monoclonal antibodies with analogues and derivatives of Pd-coproporphyrin I has revealed that the antigen is mainly located inside the antibody paratope. The protein adjoins complementary to the metalloporphyrin in such a manner that antibodies obtained discern only isomer I, and to some degree, isomer III of coproporphyrinKeywords
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