Disappearance of Phosphorylation Activity of Nucleotides from the Mitochondria-rich Cells of an Yeast,Hansenula jadinii; a Modified Pasteur Effect

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Abstract
A yeast, H. jadinii, which was 1 of the best producers of CDP-choline, lost its activity when cultured in a jar fermentor. This phenomenon was also reproduced in flasks. Cells cultured aerobically in the medium containing 1% glucose (A-cells) did not phosphorylate nucleotides although development of mitochondria was observed, but cells cultured less aerobically in the medium containing 5% glucose (D-cells) phosphorylated CMP to CTP and finally produced CDP-choline although they had only poor mitochondria. Further study revealed that A-cells were unstable in hexokinase activity although they had dense cytosol; D-cells remained stable and they had many round particles. Glycolytic activity was .apprx. 4 times stronger in D-cells than in A-cells. The phenomenon of respiration (development of mitochondria) suppressing fermentation (glycolysis) is known as the Pasteur effect. However in the present system, phosphofructokinase, the primary key enzyme of the Pasteur effect, was active in the A-cells. Therefore, this phenomenon may be a modified Pasteur effect.

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