Human Fibroblasts Grown for a Year in a Medium of Sheep Plasma and Two Solutions of Known Composition

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Abstract
In a previous article, published in this journal, it was shown that human fibroblasts can be grown for a considerable length of time in heterogeneous plasma without using any tissue extract (3). It was possible to keep such tissue growing for a period of three months, using as a medium a mixture of beef plasma and a feeding solution, the main components of which were Witte peptone, glucose, insulin, hemin, thyroxin, and cysteine.

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