Circular dichroism and proton magnetic resonance studies of dApdG modified with 2-aminofluorene and 2-acetyl-aminofluorene
- 1 November 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 1 (11) , 897-902
- https://doi.org/10.1093/carcin/1.11.897
Abstract
The conformational properties of 2′-deoxyadenylyl (3′-5′ )-2′-deoxyguanosine (dApdG) modified by the covalent binding of the carcinogens 2-acetylamino-fluorene (AAF) and 2-aminofluorene (AF) have been investigated, utilizing circular dichroism and proton magnetic resonance spectroscopy. The attachment of AF residues to the C-8 position of guanosine introduced smaller changes in the circular dichroism spectra of dApdG than the binding of AAF residues. Similarly, binding of AF residues caused lower up-field shifts for the H-2 and H-8 protons of adenine than the AAF residues.These results suggest that AF residues are less stacked with the neighboring A base in dApdG than AAF. Thus, AF residues bound on the C-8 position of guanine might induce less distortion in conformation of the modified regions than AAF residuesKeywords
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