Relation of age to isoenzyme pattern and total activity of amylase in serum.
Open Access
- 1 March 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 27 (3) , 451-454
- https://doi.org/10.1093/clinchem/27.3.451
Abstract
Pancreatic and salivary isoenzymes of amylase were determined in serum from 70 subjects. Thin-layer gel/isoelectric focusing was used to separate the isoenzymes. Because other studies (J. Lab. Clin. Med. 90: 141-151, 1977) show that the major isoamylases have isoelectric points between 5.8 and 7.2, we focused the sera on polyacrylamide gel plates with a pH gradient from 5.5 to 8.5. The separated amylase fractions were made visible by direct incubation with a commercially available dye-starch polymer. Isoelectric focusing proved to be convenient, precise, and reproducible, and it can be used as a routine analysis to detect even slight changes in serum amylase distributions. We found that the isoamylase distribution is age dependent, whereas total amylase activity shows no correlation with age.This publication has 0 references indexed in Scilit: