Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria
Open Access
- 1 January 1991
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (1) , 404-406
- https://doi.org/10.1128/jb.173.1.404-406.1991
Abstract
We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.Keywords
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