Effect of Massive Proximal Small Bowel Resection on Intestinal Brush Border Membrane Proteins in the Dog

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Abstract
Dog enterocyte brush border proteins were studied after a 75% proximal resection of the small bowel. Microvillar membrane preparations purified from ileal mucosa were sampled before and after regeneration on neighboring intestinal segments, each animal acting as its own control. After 6 wk of regeneration a statistically significant decrease of the following enzyme specific activities was observed: lactase, cellobiase, maltase, sucrase, palatinase, dextranase, trehalase, alkaline phosphatase, aminopeptidase and .gamma.-glutamyl transferase. Analysis of brush border proteins by polyacrylamide gel electrophoresis in presence of sodium dodecyl sulfate have shown after regeneration a decreased rate for the proteins with MW > 100,000 daltons. Modifications of electrophoretic patterns seem to be related to the specific activity decreases observed for brush border enzymes after regeneration, since the MW of these enzymes were found between 116,000-285,000 daltons, after gel filtration.