Association of rabbit muscle glyceraldehyde‐3‐phosphate dehydrogenase and 3‐phosphoglycerate kinase The biochemical and electron‐microscopic evidence
- 26 September 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 238 (1) , 161-166
- https://doi.org/10.1016/0014-5793(88)80248-5
Abstract
Rabbit muscle glyceraldehyde-3-phosphate dehydrogenase covalently bound to Sepharose was shown to form a complex with soluble 3-phosphoglycerate kinase. The strength of the association appeared to depend upon the functional state of both enzymes. The holoform of the dehydrogenase exhibited a lower affinity for the kinase than the enzyme-3-phosphoglycerol·NADH complex. The substrate-free 3-phosphoglycerate kinase associated much stronger with the acylated dehydrogenase than the kinase in complex with 1,3-diphosphoglycerate. Electron-microscopic evidence for the association of the soluble acyl-glyceraldehyde-3-phosphate dehydrogenase·NADH complex and 3-phosphoglycerate kinase was also obtained.Keywords
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