Adenylate Energy Charge during Batch Culture of Beneckea natriegens

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Abstract
The value of the adenylate energy charge, i.e., ([ATP]+1/2[ADP])/([ATP]+[ADP]+[AMP]), during batch culture of B. natriegens remained relatively constant during the exponential and early stationary phases of the growth cycle. During exponential growth the intracellular ATP content remained constant, the amount of ATP in the culture increasing proportionally with growth; these conditions were unaltered during growth in the presence of added cyclic AMP. On cessation of growth, significant variation in bacterial ATP content was observed depending on whether growth of the cultures terminated due to exhaustion of C or N from the medium, and on the presence or absence of added cyclic AMP.