AFRICAN SWINE FEVER .2. DETECTION OF VIRUS IN SWINE TISSUES BY MEANS OF MODIFIED DIRECT COMPLEMENT-FIXATION TEST
- 1 January 1967
- journal article
- research article
- Vol. 31 (1) , 7-+
Abstract
The modified direct complement-fixation test, supplemented with unheated normal calf serum was used to demonstrate antibodies in sera of swine immunized to African swine fever virus. These antibodies did not react in the ordinary direct non-supplemented complement-fixation test. African swine fever complement-fixing antigen in infected swine tissue is not denatured by extraction with fat solvents. Consequently, good antigens devoid of non-specific reactivity were obtained by extraction with a mixture of acetone and ether. The virus was detected in infected swine tissue harvested 1 day after beginning of pyrexia. The modified direct complement-fixation test demonstrated cross-reactions between the 6 strains of virus studied.This publication has 11 references indexed in Scilit:
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