Abstract
Several analogues of N-(5-amino-1-β-D-ribofuranosylimidazole-4-carbonyl)-L-aspartic acid 5′-phosphate (SAICAR) have been prepared by reaction of 5-amino-1-β-D-ribofuranosylimidazole-4-carboxylic acid with a number of α-amino-acids and dicyclohexylcarbodi-imide, and phosphorylation of the products. One of the derivatives of SAICAR namely N-(5-amino-1-β-D-ribofuranosylimidazole-4-carbonyl)-L-threo-β-methylaspartic acid 5′-phosphate was a competitive inhibitor of the enzyme adenylosuccinase. The mechanism of the enzyme-catalysed deamidation of SAICAR is discussed.
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