Isolation of yeast genes with mRNA levels controlled by phosphate concentration.
- 1 November 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (11) , 6541-6545
- https://doi.org/10.1073/pnas.77.11.6541
Abstract
A library of DNA from the yeast, Saccharomyces cerevisiae, was constructed in phage lambda Charon 4 vector and then screened by differential plaque filter hybridization for genes induced by phosphate starvation. Two EcoRI fragments of 7.9 and 5.0 kilobase pairs that contained such genes were isolated. These cloned fragments may each carry one of the several copies of the genes for the repressible acid phosphatase of yeast. The fragments were use to examine mRNA levels of these genes in regulatory mutants of acid phosphatase.This publication has 22 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- A comparison of two cloned mouse β-globin genes and their surrounding and intervening sequencesCell, 1978
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gelsJournal of Molecular Biology, 1977
- Analysis of chromosomal integration and deletions of yeast plasmidsNucleic Acids Research, 1977
- Hybridization of RNA to double-stranded DNA: formation of R-loops.Proceedings of the National Academy of Sciences, 1976
- A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiaeBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Genes coding for the structure of the acid phosphatases in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1975
- Regulation and Characterization of Acid and Alkaline Phosphatase in YeastJournal of General Microbiology, 1971
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966