Metachromatic Leucodystrophy: Isolation and Chemical Analysis of Metachromatic Granules
- 11 March 1966
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 151 (3715) , 1231-1233
- https://doi.org/10.1126/science.151.3715.1231
Abstract
The abnormal, cytoplasmic metachromatic granules found in the brain of metachromatic leucodystrophy were isolated in high purity. They show metachromasia by von Hirsch-Peiffer's cresyl violet method. Electron microscopy revealed very slight contaminations by other components, such as mitochondria, myelin, and ribosomes. Chemical analysis of two separately obtained collections has shown that the molar ratio of cholesterol to galactolipids to phosphatides is 1 : 1 : 1. Most of the galactolipids are sulfatides.Keywords
This publication has 9 references indexed in Scilit:
- THE LIPID COMPOSITION OF PURIFIED BOVINE BRAIN MYELIN*Journal of Neurochemistry, 1966
- IV. MEMBRANOUS CYTOPLASMIC BODIES*Journal of Neuropathology and Experimental Neurology, 1963
- A new color reaction for the quantitation of serum cholesterolClinica Chimica Acta; International Journal of Clinical Chemistry, 1960
- The quantitative analysis of plasmalogens by paper chromatographyBiochimica et Biophysica Acta, 1959
- Use of Butyl Acetate in Determination of Sialic Acid.Acta Chemica Scandinavica, 1959
- Metachromatic Form of Diffuse Cerebral SclerosisNeurology, 1957
- Quantitive estimation of sialic acidsBiochimica et Biophysica Acta, 1957
- THE QUANTITATIVE ESTIMATION OF CEREBROSIDES IN NERVOUS TISSUEJournal of Neurochemistry, 1956
- Über histologische Methoden in der Differentialdiagnose von Leukodystrophien und LipoidosenArchiv Fur Psychiatrie Und Nervenkrankheiten, 1955