The amino acid sequence of glutathione transferase from Proteus mirabilis, a prototype of a new class of enzymes
- 1 February 1993
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 211 (3) , 421-425
- https://doi.org/10.1111/j.1432-1033.1993.tb17566.x
Abstract
The complete amino acid sequence of glutathione transferase from Proteus mirabilis was determined. The sequence was reconstructed by analysis of peptides obtained after cleavage by trypsin, Glu-C and Asp-N endoproteinases. The enzyme subunit is composed of 203 amino acid residues corresponding to a molecular mass of 22856 Da. Comparison of this sequence with other known primary structures of the corresponding enzyme from different sources shows a low level of identity (17-26%) with only seven conserved residues in all the sequences considered. This novel glutathione transferase could represent the prototype of a new class, possibly including other bacterial enzymes.Keywords
This publication has 26 references indexed in Scilit:
- Mutational substitution of residues implicated by crystal structure in binding the substrate glutathione to human glutathione S-transferase πJournal of Molecular Biology, 1992
- The amino acid sequence of glutamate decarboxylase from Escherichia coliEuropean Journal of Biochemistry, 1992
- Drosophila glutathione S-transferases have sequence homology to the stringent starvation protein of Escherichia coliBiochemical and Biophysical Research Communications, 1992
- Molecular cloning and amino acid sequencing of rat liver class theta glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters of carcinogenic arylmethanolsBiochemical and Biophysical Research Communications, 1991
- Mutation of an evolutionarily conserved tyrosine residue in the active site of a human class Alpha glutathione transferaseFEBS Letters, 1991
- Purification and characterization of a novel glutathione transferase from Serratia marcescensBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
- Class π glutathione S‐transferase from pig lungEuropean Journal of Biochemistry, 1991
- N‐terminal region of Proteus mirabilis glutathione transferase is not homologous to mammalian and plant glutathione transferasesFEBS Letters, 1989
- GLUTATHIONE S-TRANSFERASES: GENE STRUCTURE, REGULATION, AND BIOLOGICAL FUNCTIONAnnual Review of Biochemistry, 1989
- Glutathione S-Transferases: Gene Structure, Regulation, And Biological FunctionAnnual Review of Biochemistry, 1989