A rapid method for the purification of antibody–enzyme conjugates
- 1 March 1979
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 57 (3) , 286-288
- https://doi.org/10.1139/o79-036
Abstract
A method is described for a rapid and efficient separation of enzyme-labeled [mouse] antibodies from the free enzyme following the coupling reaction. A single passage of the reaction mixture on a protein A-Sepharose CL-4B column gave a sharp separation of the free enzyme from the conjugate.This publication has 1 reference indexed in Scilit:
- A solid-phase enzyme immunoassay for serum ferritin.Clinical Chemistry, 1977