Fractionation of Protein Mixtures Through Differential Adsorption on a Gradient of Substituted Agaroses of Increasing Hydrophobicity
- 1 January 1975
- journal article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 5 (1) , 7-19
- https://doi.org/10.1080/00327487508061558
Abstract
Data are presented which indicate the feasibility of protein fractionation at high salt concentrations (≤ 3 M NaCl) through differential hydrophobic (non-ionic) adsorption on a series of columns of agaroses substituted with ligands of increasing hydrophobicity. By means of such a hydrophobicity gradient of connected columns, separation of mixtures of γ-globulin and serum albumin, as well as group separation of proteins in dialyzed human blood serum, has been achieved.Keywords
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