Beta 2-microglobulin is selectively associated with H-2 and TL alloantigens on murine lymphoid cells.

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Abstract
Rabbit antiserum prepared against purified rat .beta.2-microglobulin was used to immunoprecipitate molecules from lysates of radioiodinated murine thymocytes and splenocytes. All the molecules that are reactive with this serum have subunits of 44,000 and 12,000 MW, and can be identified as H-2 and TL antigens. The anti-.beta.2.mu. serum can deplete lysates of the majority of the TL and H-2 antigens which can be subsequently recognized by alloantisera. If TL and H-2 are precipitated from the lysates before the addition of anti-.beta.2.mu., no .beta.2.mu.-reactive molecules remain. Ia [immune response associated] antigens cannot be depleted from the lysates with anti-.beta.2.mu.. TL and H-2 H chains can exist as monomers and dimers. The native structure of H-2 and TL antigens is discussed.