Dependence on pH of substrate binding to lactose carrier in Escherichia coli cytoplasmic membranes
- 10 January 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 151 (1) , 102-104
- https://doi.org/10.1016/0014-5793(83)80352-4
Abstract
Lactose permease in Escherichia mediates proton-substrate cotransport. The molecular mechanism of this process is not understood. We examined the effect of proton concentration on the binding of a substance analogue to the carrier. The dissociation constant of p-nitrophenyl-α-galactoside from the carrier was dependent on pH, with an apparent pKa of 9.7Keywords
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