Lethal oxidative damage to human immunodeficiency virus by human recombinant myeloperoxidase

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Abstract
Human recombinant myeloperoxidase was evaluated in a cell‐free system for its inactivation properties on the replication of human immunodeficiency virus, HTLV‐IIIB. In the presence of a hydrogen peroxide generating system (glucose and glucose oxidase) and sodium thiocyanate, the recombinant enzyme inhibited virus‐induced syncytium formation and viral replication without causing any cytopathic effects on SupT1 reporter cells. In addition, U937 monocytoid cells, chronically infected with HIV1, were exposed to recombinant myeloperoxidase (10 U/ml) and monitored during 48 h for the accumulation of intracellular p24 viral antigen. Under these conditions, the recombinant enzyme significantly reduced intracellular viral replication without affecting cell viability.